Isoelectric focusing without carrier ampholytes.

Separation and Concentration of Proteins by Isoelectric Focusing without Carrier Ampholytes in a Nitrocellulose Membrane

This paper reports a simple isoelectric focusing (IEF) technique without carrier ampholytes to generate stable pH gradients in a nitrocellulose membrane for the fabrication of paper-based analytical devices suitable for complex biological samples. To design the custom-built IEF apparatus, silver-coated copper wire electrodes were employed; the distance between anode and cathode was 8 mm. Concen...

Comparison of Different Brands of Carrier Ampholytes for Monoclonal Antibody Charge Heterogeneity Analysis by Capillary Isoelectric Focusing

This Application Note compares the performance of four commercially available brands of wide-range pH 3 to 10 carrier ampholytes, Pharmalyte, Servalyt, HR, and SH AESlyte, for high-resolution capillary isoelectric focusing on fluorocarbon-coated capillaries. The carrier ampholytes-specific background was increased for Servalyt, and slightly increased for SH AESlyte in comparison to Pharmalyte a...

Commercial ampholytes used for isoelectric focusing may interfere with bioactivity based purification of antimicrobial peptides.

BioRad's Rotofor system has been frequently used for the purification of proteins and smaller peptides such as bacteriocins. In this study, we report that some commercially available ampholytes used with the Rotofor isoelectric focusing system possess antimicrobial activity, which may interfere with the purification of bacteriocins and bacteriocin-like substances.

Isoelectric focusing.

Capillary Isoelectric Focusing

Beale SC (1998) Capillary electrophoresis. Analytical Chemistry 70: 279. (This journal prints every two years a review on capillary electrophoresis. The article is written by an expert in the Reld and covers more or less all developments in CE including CGE of the preceding years.) Grossmann PD and Colburn JC (eds) (1996) Capillary Electrophoresis: Theory and Practice. San Diego: Academic Press...